Kasthuri Venkateswaran1, Michael Kempf1, Fei Chen1, Masataka Satomi2, Wayne Nicholson3 and Roger Kern1
1 Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
2 National Research Institute of Fisheries Science, Food Processing Division, Kanazawa-ku, Yokohama-City, Kanagawa 236-8648, Japan
3 Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA
One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA–DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, gamma-radiation, H2O2 and desiccation. The 16S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C Firmicutes. High 16S rDNA sequence similarity values were found with members of the genus Bacillus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92T and Bacillus benzoevorans DSM 5391T was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA–DNA hybridization data support the proposal of FO-92T as Bacillus nealsonii sp. nov. (type strain is FO-92T =ATCC BAA-519T =DSM 15077T).
Abbreviations: FAME, fatty acid methyl ester; JPL-SAF, Jet Propulsion Laboratory Spacecraft Assembly Facility
Source: Int J Syst Evol Microbiol 53 (2003), 165-172