Decalcification Dynamic of Dog Mineralized Tissue by Microwaves
*Dimitrius Leonardo Pitol; **Joáo Paulo Mardegan Issa; ***Flávio Henrique Caetano & ****Laurelúcia Orive Lunardi
*Graduate student, Biosciences
Institute- Molecular and Cellular Biology (UNESP), Rio Claro, Sao
** Graduate student, Faculty of Dentistry of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil.
*** Professor of
Cellular Biology, Biosciences Institute- Molecular and Cellular Biology
(UNESP), Rio Claro, Sao Paulo, Brazil.
**** Professor of Histology, Faculty of Dentistry of Ribeirao
Preto, University of Sao Paulo, Sao Paulo, Brazil.
An open access article from Int J. MorphoL, 25(2):309-313, 2007.
SUMMARY: The aim of this
article is to present the decalcification process dynamic of
mineralized tissue in dogs, teeth and jaw, comparing the traditional
decalcification method, immersion, and microwave, immersion followed by
irradiation using a domestic microwave oven, accompanying the
liberation of calcium through spectrophotometer of atomic absorption.
It was used as decalcified agent, EDTA solution or nitric acid. The
results showed that with the use of nitric acid (5%), after 15 days,
the irradiated fragments could be processed for histological analysis,
otherwise the tooth not irradiated need to be submerged for 65 days.
The EDTA decalcified action was slower than the nitric acid. The
histological observations of the irradiated samples showed an excellent
preservation of the morphological characteristics, independently of the
decalcified agent used.
KEYWORDS: Dog; Mineralized tissue; Decalcification; Microwaves; Solution.