Martiniaková M, Grosskopf B, Vondráková M, Omelka R, Fabis M.
Department of Zoology and Anthropology, Constantine the Philosopher University, 949 01-Nitra, Slovakia. firstname.lastname@example.org
purpose of this investigation was to study in detail compact bone
tissue microscopic structure of adult cows and pigs with an emphasis to
find an adequate key for the species identification. Altogether 18
femurs were analysed. Each of the bones was sectioned at the smallest
breadth of their diaphysis. Specimens were prepared using standard
histological equipment, producing thin sections approximately 80-100
mum thick. The qualitative differences between investigated species
were examined in anterior, posterior, medial and lateral views of thin
sections. The quantitative ones were counted using the specific
computer software Scion Image. We measured the area, perimeter, minimal
and maximal diameter of the Haversian canals, the Haversian systems and
the vascular canals of primary osteons. After that a discriminant
function analysis was used for the species identification. According to
our results the basic structural pattern of femur diaphysis was primary
vascular plexiform in both species. However, non-vascular bone tissue
was identified in cows and resorption lacunae were found between the
secondary osteons in pigs. The measured variables of the Haversian
canals, the Haversian systems and the primary osteon’s vascular canals
were higher in most cases in cows. Classification functions for
investigated species give a correct classification of 64.69% of cases.
This percentage value can be increased by integrating conclusions from
the qualitative analysis.
Full text available in Anat Histol Embryol. 2006 Jun;35(3):167-70.