We succeeded in regulating the growth of diatom cells on chemically modified glass surfaces. Glass surfaces were functionalized with -CF3, -CH3, -COOH, and -NH2 groups using the technique of self-assembled monolayers (SAM), and diatom cells were subsequently cultured on these surfaces. When the samples were rinsed after the adhesion of the diatom cells on the modified surfaces, the diatoms formed two dimensional arrays; this was not possible without the rinsing treatment. Furthermore, we examined the number of cells that grew and their motility by time-lapse imaging in order to clarify the interaction between the cells and SAMs. We hope that our results will be a basis for developing biodevices using living photosynthetic diatom cells.
◊ An open access article from Journal of Nanobiotechnology 2007, 5:2 distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), viewed from biologyonline.com.
Source: Umemura, K., Yamada, T., Maeda, Y., Kobayashi, K., Kuroda, R., & Mayama, S. (2007). Regulated growth of diatom cells on self-assembled monolayers. Journal of Nanobiotechnology, 5(1), 2. https://doi.org/10.1186/1477-3155-5-2