Single-chain antibody fragment (hereinafter referred to as scFv) maintains the antigen recognition specificity of parent antigen and the smallest functional antibody fragment so that its good plasticity and broad role have made it a focus of research. ScFv is a single and integrated chain. ScFv-related research has not only improved the diagnosis and treatment of viral infections but also propose a novel anti-tumor idea.
As scFv has now become more and more important in people’s daily life, it should been payed more attention, such as the applicant method. Creative Biolabs, an emerging reliable service provider in this field, shows their technique of scFv. In addition, Creative Biolabs has established a solid platform for scFv construction.
There are mainly three methods to have a scFv for the purpose of targeting a certain antigen. First step is to generate a mouse hybridoma clone and then convert full IgG into a scFv. The second step is to create an immunized phage display scFv mouse library and then utilize antigen to screen the library. The last step is to use antigen to screen a premade scFv display library, trying to get scFv clones directly. Well equipped with advanced technology, the chicken IgY can also be converted into scFv as well.
ScFv is different from the Fab fragments in a certain degree because Fab fragments not only contain the variable domains but constant regions as well. And if a monoclonal antibody is available, then the fab fragment can be created enzymatically. In this way, Fab fragments’ construction and ultimate expression are sort of complicated. Experts with years of experience from Creative Biolabs have made great efforts in antibody production, such as researching the detection method. Furthermore, as for other aspects, the same heavy and light variable chains can be used in the construction of scFv, which can be helpful in the application of Fab construction as well.
Guarantee to customers
As a reliable company, Creative Biolabs also gives customers their guarantee except for providing customers with the expression strategies and purification strategies. And if the final results do not match for 100%, the customers will not be charged.
Creative Biolabs is a leading provider in scFv construction services. It is backed up by a great team of antibody experts and advanced techniques. Visit creative-biolabs.com to learn more detailed information about scFv construction and Creative Biolabs as well.
Source : http://www.scienceplease.com/events/single-chain-antibody-fragment-scfv