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Culture of Animal Cells: A Manual of Basic Technique by R. I. Freshney

Culture of Animal Cells: A Manual of Basic Technique  

   

AUTHORS: 

  • R. Ian Freshney

PRODUCT DETAILS:

  • Hardcover: 672 pages
  • Publisher: Wiley-Liss; 5 edition (July 29, 2005)
  • Language: English
  • ISBN: 0471453293
  • Shipping Weight: 4.14 pounds


EDITORIAL REVIEWS


Book Description

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.  

Book Info

Univ. of Glasgow, UK. Training manual on the fundamental principles and techniques of animal cell culture. Includes advances, specialized techniques, biotechnology, DNA transfer, and tumor culture. Also includes new chapters on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting. Previous edition: c1994. –This text refers to an out of print or unavailable edition of this title. 

From the Back Cover

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:

  • An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
  • New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
  • An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
  • Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
  • Fully updated references
  • The clearest, most consistent presentation of step-by-step protocols available
  • Numerous diagrams, photographs, tables, and charts
  • Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. 

About the Author(s)

R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique. 

CUSTOMER REVIEWS

A SUPERB Manual of Basic Cell CultureTechnique, September 12, 2001

I purchased this book a year ago to use in a clinical virology laboratory. Although many of the recipes used in my field are essentially set, this book allowed for broad learning in the skill of cell culture. I learned many principles of cell culture that were poorly explained or not mentioned in clinical literature and books.

This book is well laid out, the chapters well written, and the information is comprehensive. Subjects include (besides the usual stuff of cell culture) chapters covering equipment materials, safety, contamination, scale up, a reagent appendix, and supplier’s listing.

This book is an excellent choice for the serious beginner. The expert will certainly find this book of value because even experts are only expert at what they do routinely and there is so much to know. This would be a great training source for research or clinical laboratories who perform cell culture.