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Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology

 Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology 

   

AUTHORS: 

  • Areles Molleman

PRODUCT DETAILS:

  • Paperback: 186 pages
  • Publisher: John Wiley & Sons (December 20, 2002)
  • Language: English
  • ISBN: 047148685X
  • Product Dimensions: 9.8 x 6.6 x 0.5 inches
  • Shipping Weight: 14.40 ounces 

EDITORIAL REVIEWS


Book Description

"Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology is a concise introduction to the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the fundamentals of the subject, platforms, equipment and environmental control. This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this important technique, including an overview of membrane biophysics, standard experimental design, data analysis and technical concerns. Aimed at novice and intermediate researchers within neurobiology laboratories, this text will also be of interest to people new to this technique, in universities and research labs as well as research technicians. A useful reference for undergraduates specialising in neurophysiology, neuropharmacology and applied physiology. Features: A self-contained guide introducing basic principles and practical applications A practical approach to the subject and written in an accessible non-technical style Extensive coverage of recording modes, analysis of results and numerous handy hints on how to avoid common pitfalls. "

From the Back Cover

Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology is a concise introduction to the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the fundamentals of the subject, platforms, equipment and environmental control. This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this important technique, including an overview of membrane biophysics, standard experimental design, data analysis and technical concerns.

Aimed at novice and intermediate researchers within neurobiology laboratories, this text will also be of interest to people new to this technique, in universities and research labs as well as research technicians. A useful reference for undergraduates specialising in neurophysiology, neuropharmacology and applied physiology.

Features:

A self-contained guide introducing basic principles and practical applications
A practical approach to the subject and written in an accessible non-technical style
Extensive coverage of recording modes, analysis of results and numerous handy hints on how to avoid common pitfalls.


CUSTOMER REVIEWS

The first of its kind, November 20, 2003

I’m giving this book a high rating because it fills a niche for which no other text exists: An absolute beginner’s guide to patch-clamping. Yes, you could read Neher & Sakmann’s hefty "Single Channel Recording" but, for the complete novice patch-clamper, it’s a bit overwhelming.

So, you walk into a patch-clamp lab, there’s a million different things on the rig and you’re feeling very confused. The microscope is much more complex than the microscope you remember from high school, there’s cabling literally everywhere, and everybody keeps mentioning how important it is not to disturb the "voodoo" shielding.

Where do you start? What do all those instruments do? Will I ever patch a cell on my own? This is the book for you.

The book does an excellent job explaining the main components on a patch-clamp rig (and advice for setting up your own if you’re just starting up a lab) and gives detailed instructions on whole-cell patch clamp techniques. (I have not evaluated the single-channel techniques but they too seem quite well written.) Also, many simple "equivalent circuit" diagrams are given so that you can understand what you’re measuring. You will patch successfully after reading this book!

My only criticism is that the book is a bit skimpy on what to do after you’ve got the cell patched. Only a few voltage-clamp techniques are explored and there is very little on experiments using dual recordings.

Otherwise, it’s an excellent book. If you’re new to patch-clamping, this is (literally) the only book for you.