Dictionary > Dideoxy sequencing

Dideoxy sequencing

Dideoxy sequencing
The most popular method of dna sequence determination (c.f. Maxam gilbert sequencing). Starting with single stranded template dna, a short complementary primer is annealed and extended by a dna polymerase. The reaction is split into 4 tubes (called a, c, g or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands.

You will also like...

Chromosome Mutations
Chromosome Mutations – I

Mutations can also influence the phenotype of an organism. This tutorial looks at the effects of chromosomal mutations, ..

Geological Periods
Geological Periods

Geological periods is a study guide that cites the different geological periods on Earth's timeline. Each has a brief ov..

The Dinosaurs

Dinosaurs represented a major turn in the evolutionary development of organisms on Earth. The first dinosaurs were presu..

Protein (illustration)
Protein Variety

The sequence of amino acids determines the type of protein. Protein is synthesized according to the sequence of nucleoti..

Crossing Over and Genetic Diversity
Inheritance and Probability

Gregor Mendel, an Austrian monk, is most famous in this field for his study of the phenotype of pea plants, including ..

Biological Cell Defense
Biological Cell Defense

Organisms employ different strategies to boost its defenses against antigens. Humans have an immune system to combat pat..