Dictionary > Dideoxy sequencing

Dideoxy sequencing

Dideoxy sequencing
The most popular method of dna sequence determination (c.f. Maxam gilbert sequencing). Starting with single stranded template dna, a short complementary primer is annealed and extended by a dna polymerase. The reaction is split into 4 tubes (called a, c, g or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands.


You will also like...

dinosaurs
The Dinosaurs

Dinosaurs represented a major turn in the evolutionary development of organisms on Earth. The first dinosaurs were presu..

Independent Assortment and Crossing Over
Independent Assortment and Crossing Over

This tutorial describes the independent assortment of chromosomes and crossing over as important events in meiosis. Read..

Transpiration
Water in Plants

The movement of molecules (specifically, water and solutes) is vital to the understanding of plant processes. This tuto..

Cell Respiration
Cell Respiration

Cell respiration is the process of creating ATP. It is "respiration" because it utilizes oxygen. Know the different stag..

Water Cycle
The Water Cycle

The water cycle (also referred to as the hydrological cycle) is a system of continuous transfer of water from the air, s..

Growth and Plant Hormones
Growth and Plant Hormones

Plants, like animals, produce hormones to regulate plant activities, including growth. They need these hormones to respo..