Dictionary > Flow cytophotometry

Flow cytophotometry

Flow cytophotometry –>
flow cytometry
(Science: technique) flow cytometry is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells.
complex computerised instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorised first by size and then computer analysed to sort the mixture of cellular elements into cell type by size.
cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser based detector system is used to excite fluorescence and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label or to gauge cell size on the basis of light scatter. The great strength of the system is that it looks at large numbers of individual cells and makes possible the separation of populations with, for example: particular surface properties.
Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilised, even when the size of the cell is identical to other subset species.
flow cytometry is a slightly imprecise but common term for the use of the fluorescence-activated cell Sorter (FACS).

You will also like...

bryophyte - sporophyte and gametophyte
Meiosis and Alternation of Generations

Plants are characterized by having alternation of generations in their life cycles. This tutorial is a review of plant m..

Growth Patterns
Growth Patterns

This tutorial describes the sigmoid curve, annual plant growth, tree growth, human growth, and insect growth as the grow..

Mātauranga Māori and Science Collaboration
Mātauranga Māori and Science

Mātauranga Māori is the living knowledge system of the indigenous people of New Zealand, including the relationships t..

Biological Energy
ATP & ADP – Biological Energy

ATP is the energy source that is typically used by an organism in its daily activities. The name is based on its structu..

Sigmund Freud and Carl Gustav Jung
Sigmund Freud and Carl Gustav Jung

In this tutorial, the works of Carl Gustav Jung and Sigmund Freud are described. Both of them actively pursued the way h..

Birth of a Human Baby
Birth of a Human Baby

Following nine months inside the mother's womb is the birth of the baby. Know the different stages of the birthing proce..