Dictionary > Ratio imaging fluorescence microscopy

Ratio imaging fluorescence microscopy

ratio imaging fluorescence microscopy
(Science: procedure) A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared.
If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically.


You will also like...

Sigmund Freud and Carl Gustav Jung
Sigmund Freud and Carl Gustav Jung

In this tutorial, the works of Carl Gustav Jung and Sigmund Freud are described. Both of them actively pursued the way h..

Balanced Diet
A Balanced Diet – Minerals and Proteins

Proteins and minerals can be derived from various dietary sources. They are essential for the proper growth and developm..

Gibberellins and Gibberellic Acid
Gibberellins and Gibberellic Acid

This tutorial describes the role of gibberellin family in plants. Find out the effects of gibberellin on plant growth an..

Thermographic image of face and neck
Regulation of Organic Metabolism, Growth and Energy Balance

The human body is capable of regulating growth and energy balance through various feedback mechanisms. Get to know the e..

Still freshwater and plants
Still Freshwater & Plants

Plants in lentic habitats have features not found in terrestrial plants. They acquired these features as they adapt to t..

A chart depicting Mendel's Law of Dominance
Mendel’s Law & Mendelian Genetics

One of Mendel’s law of inheritance is the “law of dominance”. Read this tutorial to know more about this form of i..