Dictionary > Stringency

Stringency

stringency
(Science: molecular biology) reaction conditions, notably temperature, salt, and pH that dictate the annealing of single-stranded DNA/DNA, DNA/ rNA, and RNA/RNA hybrids. at high stringency, duplexes form only between strands with perfect one-to-one complementarity, lower stringency allows annealing between strands with some degree of mismatch between bases.


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