Dictionary > Tandem

Tandem

Tandem
term used to describe multiple copies of the same sequence in a polynucleic acid that lie adjacent to one another.
Linkers are short, self-complementary synthetic oligomers which form blunt end duplexes containing a restriction endonuclease recognition sequence. They are used to create cohesive ends on a target dna molecule. Linkers may be obtained with either phosphorylated or unphosphorylated 5′ ends for use in different cloning strategies. Blunt-end ligation of phosphorylated linkers to experimental dna fragments produces concatamers of linkers at both ends of each fragment. Digestion with the appropriate restriction endonuclease creates unique cohesive ends.

Last updated on January 19th, 2021

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