Dictionary > Tandem

Tandem

Tandem
term used to describe multiple copies of the same sequence in a polynucleic acid that lie adjacent to one another.
Linkers are short, self-complementary synthetic oligomers which form blunt end duplexes containing a restriction endonuclease recognition sequence. They are used to create cohesive ends on a target dna molecule. Linkers may be obtained with either phosphorylated or unphosphorylated 5′ ends for use in different cloning strategies. Blunt-end ligation of phosphorylated linkers to experimental dna fragments produces concatamers of linkers at both ends of each fragment. Digestion with the appropriate restriction endonuclease creates unique cohesive ends.


You will also like...

Biological Cell schematic diagram
Biological Cell Introduction

It only takes one biological cell to create an organism. A single cell is able to keep itself functional through its 'mi..

Pikas are an indicator species for climate change
Indicator Species and Endangered Species

Certain species are capable of expressing characteristics indicative of the state of the ecosystem they occupy. They are..

green leaf - close up view
Leaves

Leaves are the major photosynthetic organ of a plant. Apart from that, they are also crucial to water movement. In this ..

genes controlling growth and development
Control of Growth & Development

Control of Growth & Development tutorials look at how the genetic makeup determines the biological processes on a da..

Flowers and tiny fruits of the pomegranate plant
Fruits, Flowers, and Seeds

This tutorial deals with the structure and function of flowers, fruits, and seeds. Also included here are the types of f..

sponge
Primitive Animals

Life, as we know it today, is presumed to have started in the sea and many of them were likely eukaryotic animal-like or..