Dictionary > Tandem

Tandem

Tandem
term used to describe multiple copies of the same sequence in a polynucleic acid that lie adjacent to one another.
Linkers are short, self-complementary synthetic oligomers which form blunt end duplexes containing a restriction endonuclease recognition sequence. They are used to create cohesive ends on a target dna molecule. Linkers may be obtained with either phosphorylated or unphosphorylated 5′ ends for use in different cloning strategies. Blunt-end ligation of phosphorylated linkers to experimental dna fragments produces concatamers of linkers at both ends of each fragment. Digestion with the appropriate restriction endonuclease creates unique cohesive ends.


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