(Science: technique) 1. For actinomyces, a staining method using immersion in a dark red orsellin solution in alcohol, then staining in crystal–violet solution.
See: iron haematoxylin.
2. For elastin, a staining solution of fuchsin, resorcin, and ferric chloride; elastic fibres stain blue–black.
3. For fibrin, a staining method using solutions of aniline–crystal violet and iodine–potassium iodide, then decolorizing in aniline oil and xylol; the fibrin is stained dark blue.
4. For myelin, a staining method using ferric chloride and haematoxylin; myelin stains deep blue, degenerated portions a light yellowish colour.
5. For neuroglia, a complicated process in which the final treatment is like that for staining fibrin; neuroglia and nuclei stain blue.
6. For reticulum, a silver impregnation technique in which reticulum appears as black, well-defined fibres without beading and with a relatively clear background.