Affinity chromatography
(Science: investigation) a technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance.
The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture.
Dictionary > Affinity chromatography
You will also like...
![Pollution in Freshwater Ecosystems](https://www.biologyonline.com/wp-content/uploads/2020/02/Pollution-in-Freshwater-Ecosystems-252x168.jpg)
Pollution in Freshwater Ecosystems
There are many environmental factors that arise due to the usage of water in one way or another and for every action tha..
![DNA carries genes coding for proteins](https://www.biologyonline.com/wp-content/uploads/2020/02/Genetic-information-and-protein-synthesis-224x168.jpg)
Genetic Information and Protein Synthesis
Genes are expressed through the process of protein synthesis. This elaborate tutorial provides an in-depth review of the..
![Protein (illustration)](https://www.biologyonline.com/wp-content/uploads/2020/02/Protein-Variety-300x168.jpg)
Protein Variety
The sequence of amino acids determines the type of protein. Protein is synthesized according to the sequence of nucleoti..
![Sugar Homeostasis](https://www.biologyonline.com/wp-content/uploads/2020/02/Sugar-Homeostasis-300x168.jpg)
Sugar Homeostasis
The blood sugar level is regulated by two hormones. The mechanism behind this type of negative feedback control is descr..
![Sensory systems](https://www.biologyonline.com/wp-content/uploads/2020/02/Sensory-systems-243x168.jpg)
Sensory Systems
A sensory system is a part of the nervous system consisting of sensory receptors that receive stimuli from the internal ..
![Homeostasis by water regulation](https://www.biologyonline.com/wp-content/uploads/2020/02/Homeostasis-of-Organism-Water-Regulation-252x168.jpg)
Homeostasis of Organism Water Regulation
Osmoregulation is the regulation of water concentrations in the bloodstream, effectively controlling the amount of water..