Dictionary > Complement fixation test

Complement fixation test

Definition
noun
(immunology) A form of immunological test for the detection of the presence of either a particular antibody or an antigen in a patient serum
Supplement
The complement fixation test is an immunological test wherein the binding of the complement is observed to detect the presence of an antigen or antibody as a result of their interaction. The interaction between a particular antigen and a specific antibody may either be through immune complexes (i.e. classical pathway) or by particular surfaces (i.e. alternative pathway). Thus, it is used to diagnose diseases and infections. It is particularly useful in identifying bacterial pathogens that are difficult to grow in cultures. However, ELISA and PCR methods are now preferred over complement fixation test as serological method for clinical diagnosis.
The complement fixation test is a two-step process. The antibody from the patient serum and the antigen are mixed with fresh complement. Sensitized sheep cells are then added. When the patient antibody is absent, the complement will be able to bind to the antibody-coated sheep cells and cause hemolysis. But when the antibody is present, the antigen-antibody complex binds to the complement, and therefore, no hemolysis will occur. When there is no hemolysis, it indicates a positive reaction.1
Abbreviation / Acronym: Cf test
See also:

Reference(s):

1 Terry Kotria. 2.Basic Immunology Procedure. Austin Community College. October 2010. www.austincc.edu/kotrla/SerLec2.pdf link


You will also like...

Peppered moth ("Biston betularia") melanic and light form
Examples of Natural Selection

Darwin's Finches are an example of natural selection in action. They are an excellent example of the way species' gene p..

Cell structure
Cell Structure

A typical eukaryotic cell is comprised of cytoplasm with different organelles, such as nucleus, endoplasmic reticulum, G..

Flowers and tiny fruits of the pomegranate plant
Fruits, Flowers, and Seeds

This tutorial deals with the structure and function of flowers, fruits, and seeds. Also included here are the types of f..

Biological Viruses
Biological Viruses

Viruses possess both living and non-living characteristics. This unique feature distinguishes them from other organisms...

Schematic diagram of bacterial lactose operon
Gene Action – Operon Hypothesis

Learn how the way genes control and determine every aspect of the body. This lesson uses lac operon as an example. ..

..

A chart depicting Mendel's Law of Dominance
Mendel’s Law & Mendelian Genetics

One of Mendel’s law of inheritance is the “law of dominance”. Read this tutorial to know more about this form of i..

Related Articles...

No related articles found

See all Related Topics