Dictionary > Complement fixation test

Complement fixation test

Definition
noun
(immunology) A form of immunological test for the detection of the presence of either a particular antibody or an antigen in a patient serum
Supplement
The complement fixation test is an immunological test wherein the binding of the complement is observed to detect the presence of an antigen or antibody as a result of their interaction. The interaction between a particular antigen and a specific antibody may either be through immune complexes (i.e. classical pathway) or by particular surfaces (i.e. alternative pathway). Thus, it is used to diagnose diseases and infections. It is particularly useful in identifying bacterial pathogens that are difficult to grow in cultures. However, ELISA and PCR methods are now preferred over complement fixation test as serological method for clinical diagnosis.
The complement fixation test is a two-step process. The antibody from the patient serum and the antigen are mixed with fresh complement. Sensitized sheep cells are then added. When the patient antibody is absent, the complement will be able to bind to the antibody-coated sheep cells and cause hemolysis. But when the antibody is present, the antigen-antibody complex binds to the complement, and therefore, no hemolysis will occur. When there is no hemolysis, it indicates a positive reaction.1
Abbreviation / Acronym: Cf test
See also:

Reference(s):

1 Terry Kotria. 2.Basic Immunology Procedure. Austin Community College. October 2010. www.austincc.edu/kotrla/SerLec2.pdf link


You will also like...

Still freshwater and plants
Still Freshwater & Plants

Plants in lentic habitats have features not found in terrestrial plants. They acquired these features as they adapt to t..

Biological Cell schematic diagram
Biological Cell Introduction

It only takes one biological cell to create an organism. A single cell is able to keep itself functional through its 'mi..

Plant Auxins
Plant Auxins – Phototropism & Geotropism

Plants produce hormones to regulate their growth. Auxins, for instance, influence plant growth. Know the role of auxin i..

Chemical composition of the body
Chemical Composition of the Body

The body is comprised of different elements with hydrogen, oxygen, carbon, and nitrogen as the major four. This tutorial..

Developmental Biology
Developmental Biology

Developmental biology is a biological science that is primarily concerned with how a living thing grows and attains matu..

Kidneys
Kidneys and Regulation of Water and Inorganic Ions

The kidneys are responsible for the regulation of water and inorganic ions. Read this tutorial to learn about the differ..

Related Articles...

No related articles found

See all Related Topics