Dictionary > Complement fixation test

Complement fixation test

Definition
noun
(immunology) A form of immunological test for the detection of the presence of either a particular antibody or an antigen in a patient serum
Supplement
The complement fixation test is an immunological test wherein the binding of the complement is observed to detect the presence of an antigen or antibody as a result of their interaction. The interaction between a particular antigen and a specific antibody may either be through immune complexes (i.e. classical pathway) or by particular surfaces (i.e. alternative pathway). Thus, it is used to diagnose diseases and infections. It is particularly useful in identifying bacterial pathogens that are difficult to grow in cultures. However, ELISA and PCR methods are now preferred over complement fixation test as serological method for clinical diagnosis.
The complement fixation test is a two-step process. The antibody from the patient serum and the antigen are mixed with fresh complement. Sensitized sheep cells are then added. When the patient antibody is absent, the complement will be able to bind to the antibody-coated sheep cells and cause hemolysis. But when the antibody is present, the antigen-antibody complex binds to the complement, and therefore, no hemolysis will occur. When there is no hemolysis, it indicates a positive reaction.1
Abbreviation / Acronym: Cf test
See also:

Reference(s):

1 Terry Kotria. 2.Basic Immunology Procedure. Austin Community College. October 2010. www.austincc.edu/kotrla/SerLec2.pdf link


You will also like...

Physical growth
Physical Development in Humans

This tutorial elaborates on the physical development of humans, particularly from puberty to adulthood. Read this tutori..

primitive arthropods
Arthropods

The arthropods were assumed to be the first taxon of species to possess jointed limbs and exoskeleton, exhibit more adva..

Ecological Research Measuring & Analysis
Ecological Research: Measuring & Analysis

This lesson is about the methods used for ecological research, such as quadrat and transect sampling, canopy fogging, an..

sponge
Primitive Animals

Life, as we know it today, is presumed to have started in the sea and many of them were likely eukaryotic animal-like or..

Mātauranga Māori and Science Collaboration
Mātauranga Māori and Science

Mātauranga Māori is the living knowledge system of the indigenous people of New Zealand, including the relationships t..

genes controlling growth and development
Control of Growth & Development

Control of Growth & Development tutorials look at how the genetic makeup determines the biological processes on a da..