Dictionary > Dna ligase

Dna ligase

Dna ligase
(Science: enzyme molecular biology) enzyme involved in dna replication. The dna ligase of E. Coli seals nicks in one strand of double stranded dna, a reaction required for linking precursor fragments during discontinuous synthesis on the lagging strand. Nicks are breaks in the phosphodiester linkage that leave a free 3- OH and 5- phosphate. The ligase from phage t4 has the additional property of joining two dna molecules having completely base paired ends. Dna ligases are crucial in joining dna molecules and preparing radioactive probes (by nick translation) in recombinant DNA Technology.
An enzyme that promotes the joining up of adjacent bonds in a polynucleotide Chain, which is used in gene.


You will also like...

Stem
Stems

Stems primarily provide plants structural support. This tutorial includes lectures on the external form of a woody twig ..

Growth and Plant Hormones
Growth and Plant Hormones

Plants, like animals, produce hormones to regulate plant activities, including growth. They need these hormones to respo..

mRNA
Protein Synthesis

Part of the genetic information is devoted to the synthesis of proteins. mRNA, a type of RNA, is produced as a transcri..

river
Running Water Freshwater Communities

This tutorial introduces flowing water communities, which bring new and dithering factors into the equation for possible..

Plant meristem
Plant Meristems and Growth

In plants, growth occurs in meristems, which are the site of repeated cell division of unspecialized cells. These cells ..

Freshwater aquatic plankton
Freshwater Communities & Plankton

Planktons are microscopic organisms that live suspended in aquatic habitats. There are two groups: the phytoplanktons an..