Dictionary > Maxam-Gilbert sequencing

Maxam-Gilbert sequencing

Definition
noun
A DNA sequencing technique developed in 1976-1977 by Allan Maxam and Walter Gilbart to identify the sequence of nucleotide bases of a nucleic acid molecule by means of preferential, base-specific methylation (using dimethyl sulfate) and hydrazinolysis
Supplement
This method involves DNA purification procedures and radioactive labelling at one end of the DNA to be sequenced. The molecule will next be treated with chemicals, each cleaving at specific bases (i.e. adenine, guanine, cytosine, or thymine). The result willl be a series of varying sizes of radioactive-labelled DNA fragments. These fragments will then be separatedthrough gel electrophoresis. The smallest fragments will travel farthest while the largest fragments will be nearest to the gel well. Thus, the sequence of nucleotide bases may be determined through the marks that the labelled fragments made on the gel.
It was once of the most utilized method of DNA sequencing. However, it became less favoured when more advanced sequencing methods came about owing to their less technical complexity, less use of health-hazard chemicals, and higher capability for large scale sequencing.


You will also like...

Cells in different stages of meiosis
Meiosis – The Genetics of Reproduction

Meiosis is a form of cell division that creates gametes. It is comprised of two divisions that in the end, the resulting..

Peppered moth ("Biston betularia") melanic and light form
Examples of Natural Selection

Darwin's Finches are an example of natural selection in action. They are an excellent example of the way species' gene p..

Oligodendrocyte and Schwann cell
The Central Nervous System

Myelin sheath is essential for a faster conductivity of signals. Know more about this feature of some neurons in the Cen..

Ecological Research Measuring & Analysis
Ecological Research: Measuring & Analysis

This lesson is about the methods used for ecological research, such as quadrat and transect sampling, canopy fogging, an..

Crossing Over and Genetic Diversity
Inheritance and Probability

Gregor Mendel, an Austrian monk, is most famous in this field for his study of the phenotype of pea plants, including ..

Adaptation Tutorial
Adaptation Tutorial

Adaptation, in biology and ecology, refers to the process or trait through which organisms or the populations in a habit..

Related Articles...

No related articles found

See all Related Topics