Dictionary > Recombinant DNA

Recombinant DNA

Definition
noun
Genetically-engineered DNA molecule formed by splicing fragments of DNA from a different source or from another part of the same source, and then introduced into the recipient (host) cell
Supplement
Recombinant DNAs are molecules of DNA that are formed through genetic recombination methods. DNA molecules from two or more different sources are cleaved using restriction enzymes and then joined together using ligases. Recombinant DNA is possible since the fundamental chemical structure of DNA molecules is the same in most organisms.
The DNA fragments may come from any species. Thus, recombinant DNA is sometimes referred to as chimeric DNA due to the DNA resulting from the recombination of DNA fragments from two different species, likened to a chimera. An example of this is a plant DNA that is joined to a bacterial DNA.
The different methods used to produce recombinant DNA are (1) transformation, (2) phage introduction, and (3) non-bacterial transformation. The protein expressed from a recombinant DNA us termed recombinant protein. The expression into protein though would require restructuring of the gene that would produce a particular mRNA molecule, which can be used by the translational apparatus (e.g. promoter, translational initiation signal, and transcriptional terminator) within the host cell.1
Abbreviation / Acronym: rDNA
Synonym:

  • chimeric DNA

See also:

  • DNA
  • cloning
  • recombinant DNA technology
  • Reference(s):

    1 Hannig, G. and Makrides, S. (1998). “Strategies for optimizing heterologous protein expression in Escherichia coli”. Trends in Biotechnology 16 (2): 54–60.


    You will also like...

    Salmon jumping upstream in the river
    Lotic Communities & Animals

    A running water environment offers numerous microhabitats for many types of animals. Similar to plants, animals in lotic..

    Principles of Hormonal Control Systems
    Principles of Hormonal Control Systems

    Hormones are essential in the regulation of the activity of the various biological systems of the human body. The ineffi..

    sponge
    Primitive Animals

    Life, as we know it today, is presumed to have started in the sea and many of them were likely eukaryotic animal-like or..

    Buttress roots
    Roots

    This study guide tackles plant roots in greater detail. It delves into the development of plant roots, the root structur..

    Types and Causes of Brain Damage
    Types and Causes of Brain Damage

    This tutorial describes the different types and causes of brain damage. Find out how genetics, physical injury, lack of ..

    Ploidy
    Polyploidy

    Humans are diploid creatures. This means that for every chromosome in the body, there is another one to match it. Howeve..