Dictionary > Replica plating

Replica plating

replica plating
(Science: technique) technique for testing the genetic characteristics of bacterial colonies. A dilute suspension of bacteria is first spread, in a petri dish, on agar containing a medium expected to support the growth of all bacteria, the master plate. Each bacterial cell in the suspension is expected to give rise to a colony. A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony. The bacteria can then be stamped onto new sterile petri dishes, plates, in the identical arrangement. The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics. Thus colonies can be identified that cannot grow without specific nutrients or that are antibiotic resistant and cells with mutations in particular genes can be isolated.


You will also like...

Stem
Stems

Stems primarily provide plants structural support. This tutorial includes lectures on the external form of a woody twig ..

Endemic Flora of New Zealand
New Zealand’s Unique Flora

If New Zealand has lots of unique animals, it's also got a whole lot of unique plants. Find out more about some of them,..

Control of body movement
Control of Body Movement

Some of the body movements can be controlled at will, others cannot. The body has a motor program, which is the pattern ..

Plant biology
Plant Biology

Plantlife can be studied at a variety of levels, from the molecular, genetic and biochemical level through organelles, c..

Biosecurity and Biocontrol
Biosecurity and Biocontrol

This lesson explores the impact of biosecurity threats, and why they need to be identified and managed. Examples to incl..

Golgi apparatus (3-D illustration)
Role of Golgi Apparatus & Endoplasmic Reticulum in Protein Synthesis

The endoplasmic reticulum and Golgi apparatus are the organelles involved in the translation step of protein synthesis a..

Related Articles...

No related articles found

See all Related Topics