Dictionary > Sticky end

Sticky end

Definition
noun, plural: sticky ends
(molecular biology) A fragment of DNA (often produced by a staggered cut on the DNA using restriction enzymes) in which the terminal portion has a stretch of unpaired nucleotides, and the strands are not of the same length.
Supplement
The ends of the DNA are sticky because it can easily match up with a complementary overhang.
For example, these two sticky ends can be joined together since they can form complementary base pairs in the overhang region:
5′-ATCTGACT ——+ GATGCGTATGCT-3′

3′-TAGACTGACTACG——–CATACGA-5′
Synonym: DNA end, cohesive end.

Compare: blunt end.

See also: restriction enzyme, overhang (molecular biology).


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