Articles > Substrate definition – quiz Substrate definition – quiz Quiz Choose the best answer. 1. A substrate refers to the substratum where organisms live or attach to.TrueFalse 2. In biochemistry, a substrate is a substance that is acted upon by an enzyme.TrueFalse 3. A chemical bond forms between the active site of the enzyme and the substrate, forming an enzyme-substrate complexTrueFalse 4. Carbohydrate is an example of a biochemical substrate.TrueFalse 5. The phenomenon via which the enzymes activate the specified substrates is called substrate specificity.TrueFalse Send Your Results (Optional) Your Name To Email Time is Up!