Articles > Substrate definition – quiz Substrate definition – quiz Quiz Choose the best answer. 1. A substrate refers to the substratum where organisms live or attach to. True False 2. In biochemistry, a substrate is a substance that is acted upon by an enzyme. True False 3. A chemical bond forms between the active site of the enzyme and the substrate, forming an enzyme-substrate complex True False 4. Carbohydrate is an example of a biochemical substrate. True False 5. The phenomenon via which the enzymes activate the specified substrates is called substrate specificity. True False Send Your Results (Optional) Your Name To Email Time's up