Dictionary > Basic dye

Basic dye

Definition
noun, plural: basic dyes
(biological techniques) A dye that ionize in solution giving a positively-charged ions and is used to produce a brilliant color during staining of biological specimen
Supplement
Biological staining is a common laboratory technique applied in the study and identification of a specimen. It is carried out for use in microscopy. An unstained specimen would be difficult to observe in a typical microscope. Staining or dyeing the specimen helps in highlighting microscopic structures such as cell organelles, cells and tissues. There are different kinds of staining techniques. Examples include gram staining, endospore staining, counterstaining, differential staining, Ziehl-Neelsen stain, Haematoxylin and eosin (H&E) staining, Papanicolaou staining, PAS staining, Masson’s trichrome, Romanowsky stains, silver staining, Sudan staining, and Conklin’s staining. Dyes that are used for biological staining may be basic, neutral, or acid.
Basic dyes used in biological staining ionize in solution to give cations (positively charged ions). The cation in a basic dye is the colored component of the dye molecule that binds to anionic groups of nucleic acids or acidic mucopolysaccharides. Basic dyes stain basophilic structures such as nuclei, ribosomes and GAGs. Examples of basic dyes are methylene blue, toluidine blue, thionine, and crystal violet.
Synonym(s):

  • basic stain

Compare:

  • acid dye
  • neutral stain
  • See also:

    Mentioned in:

  • Basoplasm
  • Neutrophilous
  • Basophil adenoma


    You will also like...

    human brain structure
    The Conscious & Unconscious Nervous System

    This tutorial elaborates on how the nervous system works, particularly at the tissue level of the brain. There are three..

    Ecosystem Succession
    Ecosystem Succession

    If the balance of nature is left untouched, landscapes can change dramatically over time. A previous ecosystem is supers..

    kinesin motor proteins transporting molecules across microtubules
    Movement of Molecules Across Cell Membranes

    Molecules move within the cell or from one cell to another through different strategies. Transport may be in the form of..

    Community Patterns

    Learn about community patterns and the ecological factors influencing these patterns. Revisit some of the ecosystems you..

    Fossil trilobite imprint in the sediment
    Insects

    There are more species of insects than any other species combined. This surely illustrates that insects have the selecti..

    Disturbance on a still water
    Abiotic Factors – Water Conditions

    A still body of water may be disturbed by a variety of factors. One of them is wind. In fact, it is considered as the pr..