Dictionary > Plaque assay

Plaque assay

Definition
noun
An assay used for virus isolation and purification, and to determine viral titers.
Supplement
The plaque assay is originally a virological assay developed to count and measure infectivity of bacteriophages. Later, it was applied to count mammalian viruses as well. The plaque assay remains to be the most widely used technique for virus isolation and purification, and to determine viral titers. The basis of the technique is to measure the ability of a single infectious virus to form a “plaque” on a confluent monolayer culture of cells. A plaque is formed as a result of infection of one cell by a single virus particle followed by the replication of that virus, and eventually, the death of the cell. The newly replicated virus particles will then infect and kill surrounding cells. The culture will then be stained with a dye, which stains only viable cells but not the dead cells. Hence, the dead cells in the plaque will appear unstained against the colored background.
Nowadays, the plaque assay is applied to detect cells producing antibodies that destroy erythrocytes (by hemolysis). The basis is the clear plaque that indicates the red blood cells have been hemolysed by the antibodies.


You will also like...

Homeostasis by water regulation
Homeostasis of Organism Water Regulation

Osmoregulation is the regulation of water concentrations in the bloodstream, effectively controlling the amount of water..

Freshwater Ecology
Freshwater Ecology

Freshwater ecology focuses on the relations of aquatic organisms to their freshwater habitats. There are two forms of co..

Lotic community of algal species
Lotic Communities & Algae

Lotic communities have conditions that are rather harsh for typical plants. Thus, the diversity of plant species in loti..

Gene Regulation in Eukaryotes
Gene Regulation in Eukaryotes

Learn about the general structure of a eukaryotic gene, the transcription factors, and post-transcriptional regulation....

Mātauranga Māori and Science Collaboration
Mātauranga Māori and Science

Mātauranga Māori is the living knowledge system of the indigenous people of New Zealand, including the relationships t..

primitive arthropods
Arthropods

The arthropods were assumed to be the first taxon of species to possess jointed limbs and exoskeleton, exhibit more adva..