Dictionary > Electroporation

Electroporation

Definition
noun, plural: electroporations
A non-chemical method that transfers the genetic material into the recipient cell through an electrical field that increases cell membrane permeability
Supplement
Electroporation is a useful molecular biology technique that enables transformation of bacteria and yeasts, and transfection of tissue culture cells (e.g. mammalian cells) through non-chemical means. It uses short pulses of an intense electric field to cells. The electrical field increases the permeability of the cell membrane. As a result, chemicals such as drugs and genetic material can pass through the otherwise impermeable cell membrane. Electroporation is a preferred method to introduce foreign genes to target cells because its convenience and efficacy. 1
The term electroporation comes from the fact that subjecting the cell membrane to high-voltage electric shocks leads to the formation of pores. These pores are sufficiently large to permit the passage of macromolecules into and out of the cell. The pores will eventually close by a natural decay process that is delayed at 0°C.
Synonym(s):

  • electropermeabilization

See also:

  • transfection
  • gene
  • genetic material
  • cell squeezing
  • sonoporation
  • optical transfection
  • protoplast fusion
  • impalefection
  • hydrodynamic delivery
  • Reference(s):

    1Neumann, E; Schaefer-Ridder, M; Wang, Y; Hofschneider, PH (1982). “Gene transfer into mouse lyoma cells by electroporation in high electric fields”. The EMBO Journal 1 (7): 841–5. PMC 553119.

    2Potter, H., & Heller, R. (2003). Transfection by Electroporation. Current Protocols in Molecular Biology / Edited by Frederick M. Ausubel … et Al.,CHAPTER, Unit–9.3. doi:10.1002/0471142727.mb0903s62


    You will also like...

    primitive arthropods
    Arthropods

    The arthropods were assumed to be the first taxon of species to possess jointed limbs and exoskeleton, exhibit more adva..

    Human Neurology
    Human Neurology

    Human Neurology deals essentially with the nervous system of humans. It also features the various theories put forward b..

    Human gastrointestinal tract
    Human Biology – Food and Digestion

    This tutorial recognizes the importance of food as a source of energy that will fuel many biological processes. A good d..

    Ploidy
    Polyploidy

    Humans are diploid creatures. This means that for every chromosome in the body, there is another one to match it. Howeve..

    green leaf - close up view
    Leaves

    Leaves are the major photosynthetic organ of a plant. Apart from that, they are also crucial to water movement. In this ..

    Human Reproduction
    Human Reproduction

    Humans are capable of only one mode of reproduction, i.e. sexual reproduction. Haploid sex cells (gametes) are produced ..

    Related Articles...

    No related articles found

    See all Related Topics