Dictionary > Sonoporation

Sonoporation

Definition
noun
A mechanical method of delivering molecules into the cell using sound, e.g. ultrasonic frequencies
Supplement
Sonoporation is one of the non-chemical means being used to transfer molecules into the target cell; others are electroporation, impalefection, optical transfection, and hydrodynamic delivery. Similar to these non-chemical means, sonoporation increases the permeability of cell membrane to macromolecules such as DNA. It does so by creating transient pores in cell membranes by subjecting the recipient cell to sonication. Typically, the sound used to bring about the formation of pores in cell membrane is ultrasonic frequencies. Sonoporation, thus, employs acoustic cavitation of microbubbles interacting with nearby cell membranes that consequently enables the transfer of macromolecules into the recipient cells.1 When employing sonoporation for transfection or transformation of cells, cellular viability must be considered because prolonged exposure to low-frequency ultrasound may result in the rupturing, and ultimately to the death, of the cell.
Sonoporation is being considered for use in gene therapy (in vivo). As a medical treatment, it may be used to deliver modified DNA into specific recipient cells via ultrasound frequencies.
Synonym(s):

  • cellular sonication

See also:

  • transfection
  • gene
  • genetic material
  • electroporation
  • cell squeezing
  • optical transfection
  • protoplast fusion
  • impalefection
  • hydrodynamic delivery
  • Reference(s):

    1Yizhi Song; Garaventa, G; Bottero, S; Leprini, AE; Pallestrini, E; Castello, E; Pallestrini, EA; Huang, W. E. (2007). “Ultrasound-mediated DNA transfer for bacteria”. Nucleic Acids Res. 35 (19): 1073–80. doi:10.1093/nar/gkm710.


    You will also like...

    Freshwater Ecology
    Freshwater Ecology

    Freshwater ecology focuses on the relations of aquatic organisms to their freshwater habitats. There are two forms of co..

    Balanced Diet
    A Balanced Diet – Minerals and Proteins

    Proteins and minerals can be derived from various dietary sources. They are essential for the proper growth and developm..

    Golgi apparatus (3-D illustration)
    Role of Golgi Apparatus & Endoplasmic Reticulum in Protein Synthesis

    The endoplasmic reticulum and Golgi apparatus are the organelles involved in the translation step of protein synthesis a..

    Bryophytes
    Bryophytes

    Bryophytes (nonvascular plants) are a plant group characterized by lacking vascular tissues. They include the mosses, th..

    Freshwater lake ecosystem
    Freshwater Producers and Consumers

    Freshwater ecosystem is comprised of four major constituents, namely elements and compounds, plants, consumers, and deco..

    genes controlling growth and development
    Control of Growth & Development

    Control of Growth & Development tutorials look at how the genetic makeup determines the biological processes on a da..

    Related Articles...

    No related articles found

    See all Related Topics