Dictionary > Reverse passive agglutination

Reverse passive agglutination

Definition
noun
A type of agglutination reaction in which known antibody is bound to a carrier particle instead of the antigen
Supplement
Agglutination, or the clumping together of cells, could be used in the identification of bacterial species by means of identifying the antigen specific to a certain antibody. Agglutination may be active or passive depending on the method of bringing about agglutination reaction. In active agglutination, there is no need for wrapping the antigen with an inert particle prior to agglutination. In passive agglutination, the agglutination reaction would have to make the antigen bound to an inert or carrier particle such as latex beads and tanned erythrocytes before it is made to react with a particular antibody. However, there is another biological method in which the inert or carrier particle is bound not to an antigen but to a known antibody. This is referred to as reverse passive agglutination. In this process, the antibody must be oriented in such a way that the active site is facing outward. It is used in the detection of microbial antigens such as Group A and B Streptococcus, Staphylococcus aureus, Neisseria meningitides, Haemophilus influenza, Cryptococcus neoformans, Mycoplasma pneumonia, and Candida albicans. 1
See also:

Related term(s):

Reference(s):

1 Terry Kotria. 2.Basic Immunology Procedure. Austin Community College. October 2010. www.austincc.edu/kotrla/SerLec2.pdf link


You will also like...

Water Cycle
The Water Cycle

The water cycle (also referred to as the hydrological cycle) is a system of continuous transfer of water from the air, s..

Pink Lotus plant on a pond
Freshwater Lentic Communities & Animals

This tutorial looks at some of the communities in freshwater lentic habitats. For instance, symbiosis occurs in a commun..

Evolution of Life - Ancient Earth
Evolution of Life – Ancient Earth

Autotrophs flourished, absorbing carbon and light. Soon after, primitive life forms that could assimilate oxygen thrived..

Angle Oak Tree
Seed Plants

Seed plants are vascular plants. They differ from the other vascular plants in producing seeds that germinate into a new..

Human Nervous System
The Human Nervous System

The nervous system is essentially a biological information highway. This tutorial gives an overview of the nervous syste..

Community Patterns

Learn about community patterns and the ecological factors influencing these patterns. Revisit some of the ecosystems you..

Related Articles...

No related articles found

See all Related Topics