Dictionary > Reverse passive agglutination

Reverse passive agglutination

Definition
noun
A type of agglutination reaction in which known antibody is bound to a carrier particle instead of the antigen
Supplement
Agglutination, or the clumping together of cells, could be used in the identification of bacterial species by means of identifying the antigen specific to a certain antibody. Agglutination may be active or passive depending on the method of bringing about agglutination reaction. In active agglutination, there is no need for wrapping the antigen with an inert particle prior to agglutination. In passive agglutination, the agglutination reaction would have to make the antigen bound to an inert or carrier particle such as latex beads and tanned erythrocytes before it is made to react with a particular antibody. However, there is another biological method in which the inert or carrier particle is bound not to an antigen but to a known antibody. This is referred to as reverse passive agglutination. In this process, the antibody must be oriented in such a way that the active site is facing outward. It is used in the detection of microbial antigens such as Group A and B Streptococcus, Staphylococcus aureus, Neisseria meningitides, Haemophilus influenza, Cryptococcus neoformans, Mycoplasma pneumonia, and Candida albicans. 1
See also:

Related term(s):

Reference(s):

1 Terry Kotria. 2.Basic Immunology Procedure. Austin Community College. October 2010. www.austincc.edu/kotrla/SerLec2.pdf link


You will also like...

Direct and indirect physiologic effects of growth hormone
Animal Growth Hormones

Hormones are produced in the endocrine glands of animals. The pituitary gland and hypothalamus are the most impor..

Cell structure
Cell Structure

A typical eukaryotic cell is comprised of cytoplasm with different organelles, such as nucleus, endoplasmic reticulum, G..

dinosaurs
The Dinosaurs

Dinosaurs represented a major turn in the evolutionary development of organisms on Earth. The first dinosaurs were presu..

Structural depiction of catalase, an enzyme
Protein Activity and Cellular Metabolism

Proteins have a crucial role in various biological activities. Get to know how proteins are able to perform as enzymes, ..

primitive arthropods
Arthropods

The arthropods were assumed to be the first taxon of species to possess jointed limbs and exoskeleton, exhibit more adva..

Lentic community
Freshwater Communities & Lentic Waters

Lentic or still water communities can vary greatly in appearance -- from a small temporary puddle to a large lake. The s..

Related Articles...

No related articles found

See all Related Topics